Multicolor Flow Cytometry Panel Design

Follow these guidelines and recommendations to achieve optimized panels.

Multicolor flow cytometry panel design.

Use a tool like the molecular probes fluorescence spectraviewer to visualize the spectral overlap of fluorophores. Know your instrument and the fluorophores that you can use. Keep adding until you have all the markers you want. Panel design and multicolor flow cytometry information advances in both flow cytometry reagents and instrumentation allow researchers to run increasingly complex multicolor experiments.

To find more information check your cytometer manual view our instrument guide page or check with your local flow core facility. Constructing multicolor panels for flow cytometry can be a challenging task. Know the configuration of the instrument being used laser and filters before you begin. However increasing the number of antigens and fluorochromes also increases the complexity of the experimental design.

Flow cytometry multicolour panel design well planned and executed assays can allow the detection and monitoring of multiple parameters simultaneously in complex samples. Such multicolour assays can produce an enormous amount of data from precious or limited samples. When designing a multicolor flow cytometry panel there are several key points to consider. Build multicolor flow cytometry panels in just a few simple steps start your design by first selecting your instrument from the drop down menu or create a customized instrument to build a personalized panel select the target and species you want to identify.

Empower your multicolor panel design with bd tools and information assembled here to help you take full advantage of the power of multicolor flow cytometry. Panel design education at bd we provide innovative instruments reagents and software to support scientists throughout the full continuum of flow cytometry experiments. Flow cytometry is a powerful tool for identifying and analyzing multiple antigens simultaneously.